Highly Efficient In Vitro Site-Specific Recombination System Based on Streptomyces Phage φBT1 Integrase
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چکیده
منابع مشابه
Control of Directionality in Streptomyces Phage φBT1 Integrase-Mediated Site-Specific Recombination
Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate double strand breakage and exchange. A recombination directionality factor (RDF) is commonly required for switching integration to excision. Here...
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Vol. 40, No. 4 (2006) BioTechniques 1 Supplementary Figure S1. Structure of constructs used and representation of FRT locus. (A) Plasmids used to detect the Bxb1 integrase-mediated sitespecific recombination. CMVp, SV40t, Luc, stop, and Hygro are cytomegalovirus promoter (CMVp), simian virus 40 terminator (SV40t), luciferase gene, transcription termination sequence, and hygromycin resistance ge...
متن کاملDNA cleavage is independent of synapsis during Streptomyces phage phiBT1 integrase-mediated site-specific recombination.
Bacteriophage-encoded serine recombinases have great potential in genetic engineering but their catalytic mechanisms have not been adequately studied. Integration of ϕBT1 and ϕC31 via their attachment (att) sites is catalyzed by integrases of the large serine recombinase subtype. Both ϕBT1 and ϕC31 integrases were found to cleave single-substrate att sites without synaptic complex formation, an...
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Background: PhiC31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. This system enables integration of exogenous DNA into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene. Objectives: Identification of a novel pseudo attP site. Materials and Methods...
متن کاملGenome engineering and direct cloning of antibiotic gene clusters via phage ϕBT1 integrase-mediated site-specific recombination in Streptomyces
Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting of a specialized recombinase and its target sites. In this study, a novel strategy based on phage ϕBT1 integrase-mediated site-specific recombination was developed, and used for simultaneous Streptomyces gen...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2008
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00777-08